Blood plasma isn’t just another biopharma input—it’s a biological treasure. It contains rare and life-saving proteins like immunoglobulins, albumin, and clotting factors. These molecules can’t be synthetically manufactured at scale—once damaged, they’re gone for good. That’s why each step of fractionation demands not just precision, but extraordinary gentleness.

Fractionation: A Delicate, Multistep Journey

Metenova’s own blog underscores the extreme care built into each stage of plasma processing. Here’s a closer look:

1. Collection & Freezing
   Plasma is collected—typically via plasmapheresis—and rapidly frozen (≤−30 °C) to preserve labile proteins and inhibit degradation.

2. Cryoprecipitation
   Thawed slowly at 1–6 °C, plasma forms a cryoprecipitate rich in fibrinogen, Factor VIII, von Willebrand factor (vWF), and fibronectin—key therapeutic proteins.

3. Cryosupernatant Processing
   The remaining liquid—cryosupernatant—is often concentrated via ultrafiltration or controlled evaporation to improve processing efficiency before further purification.

4. Ethanol Fractionation / Cohn Process
   This cold ethanol method leverages changes in ethanol concentration, pH, and temperature to sequentially precipitate, separate, and ultimately isolate specific proteins like albumin.

5. Advanced Purification
   Steps like depth filtration, chromatography, viral inactivation, and final sterile formulation fine-tune purity and safety—ultimately preparing proteins for therapeutic use.

Throughout these stages, mixing must remain precise, uniform, and extraordinarily gentle. Any excess friction, heat, or mechanical stress can damage proteins, degrade yield, and risk product safety.

Where Traditional Mixers Fall Short

Conventional magnetic mixers—though common in biopharma—often rely on bearings or contact points. Even minimal friction or heat from these contact surfaces can:

• Denature fragile plasma proteins

• Trap or shed particulates that clog filters

• Compromise downstream steps like chromatography or viral inactivation

In plasma fractionation, “good enough” mixing simply isn’t. When every milligram and molecule matters, the mixer’s performance becomes mission-critical. Meanwhile, major brands in this space are discontinuing their product lines.

Why Truelev Excels: Designed for Molecular Sensitivity

Metenova’s Truelev mixer stands apart by eliminating contact entirely. With full magnetic levitation, there’s:

• No mechanical friction or bearing load

• Zero heat generation at the mixing interface

• No wear or particle shedding

• Complete preservation of protein integrity

This results in a mixing environment that’s smooth, sterile, and safe—protecting sensitive proteins from cryoprecipitate to ethanol precipitation, all the way to final formulation.

From Theory to Real-World Impact

Processors using Truelev report:

• Improved protein yield due to minimized denaturation

• Less filter clogging and aggregation, extending filter life

• Reduced batch failures

• More consistent product quality

• Virtually zero maintenance—critical in sterile, high-throughput environments

Truelev: The Right Tool for the Hardest Challenge

In our earlier blogs, we discussed how Zero‑G transformed hygienic mixing through magnetic coupling—and then how Truelev protected biologics during high‑value transfers. Plasma fractionation is the ultimate test: a process where every molecule, motion, and moment matters. Truelev doesn’t just meet that standard—it defines it.

For processes where stress isn’t an option, Truelev isn’t just better—it’s essential.